Coordinators: D.W. Heermann, M. Salmhofer, U. Schwarz, M. HaverkortThursdays 14-16 o'clock
Institute for Theoretical Physics
Summer Term 2018 Schedule
To be confirmed:
- Mario Nicodemi, University of Nepals and Max Dellbruck Center Berlin
- Thu 17.05.18 at 14 c.t.
Pierre Gönczy EPFL Lausanne
Mechanisms of centriole assembly
The centriole is a remarkable microtubule-based organelle that is essential for the formation of cilia, flagella and centrosomes. The centriole is organized around a nine-fold symmetrical cartwheel typically ~100 nm in height, which is critical for the onset of organelle biogenesis. The cartwheel comprises a stack of ring-containing entities that each accommodates nine homodimers of SAS-6 proteins. In contrast to the knowledge about the self-assembly properties of SAS-6 proteins, the mechanisms enabling ring stacking are poorly understood. Furthermore, the assembly dynamics of SAS-6 ring-containing entities remains elusive. After introducing the subject matter, I will report notably on our development of a cell-free assay to address this important open question using the Chlamydomonas reinhardtii SAS-6 protein CrSAS-6. Using high-speed atomic force microscopy (AFM)-based, we monitored the assembly dynamics of CrSAS-6 homodimers, and thus determined possible routes and kinetic rates for ring formation.